DNA damage in Molt-4 T-lymphoblastoid cells exposed to cellular telephone radiofrequency fields in vitro

Ž w Molt-4 T-lymphoblastoid cells have been exposed to pulsed signals at cellular telephone frequencies of 813.5625 MHz iDEN . Ž . Ž y1 w signal and 836.55 MHz TDMA signal . These studies were performed at low SAR averages2.4 and 24 mW g for iDEN and 2.6 y1 . and 26 mW g for TDMA in studies designed to look for athermal RF effects. The alkaline comet, or single cell gel electrophoresis, Ž . assay was employed to measure DNA single-strand breaks in cell cultures exposed to the radiofrequency RF signal as compared to concurrent sham-exposed cultures. Tail moment and comet extent were calculated as indicators of DNA damage. Statistical differences in the distribution of values for tail moment and comet extent between exposed and control cell cultures were evaluated with the Kolmogorov–Smirnoff distribution test. Data points for all experiments of each exposure condition were pooled and analyzed as single . w y1 groups. It was found that: 1 exposure of cells to the iDEN signal at an SAR of 2.4 mW g for 2 h or 21 h significantly decreased . y1 DNA damage; 2 exposure of cells to the TDMA signal at an SAR of 2.6 mW g for 2 h and 21 h significantly decreased DNA damage; . w y1 . 3 exposure of cells to the iDEN signal at an SAR of 24 mW g for 2 h and 21 h significantly increased DNA damage; 4 exposure of cells to the TDMA signal at an SAR of 26 mW g for 2 h significantly decreased DNA damage. The data indicate a need to study the effects of exposure to RF signals on direct DNA damage and on the rate at which DNA damage is repaired. q 1998 Elsevier Science S.A.